More Weather Delays

The weather has been very uncooperative for the past week and a half! It was hard getting everyone back into town after our field work last week, because the weather was bad and the helo's couldn't fly. Katie, Elizabeth, and Ross got stuck out at Lake Fryxell camp for an extra day because no helo could get into the Valley to pick them up. I kept trying to leave McMurdo to go to Lake Bonney, but that trip kept getting canceled. We finally made it to our destinations, after a couple days of delay. We all made it back to McMurdo on Tuesday, after completing most of the work we needed to do last week. Since then, we've all been back in McMurdo processing samples in the lab. Today, we are once again trying to get back to the field. We were all supposed to fly out this morning to F6 camp on Lake Fryxell. But, it's snowing again, and you can only see about 200 m across McMurdo Sound, so we're not going anywhere!

Since we're all stuck here in town, we decided to have a luau on the Florida Keys (after my walk to the post office to pick up some super fun packages that were sent to me). We decorated ourselves using my Luau in an Envelope kit. And we basked on the edge of the miniature beach that was sent to me from Florida (which I'm holding in my hands). Here we are partying in the hallway in the science building: Karen, Katie, Diana, Breana, Uffe, me, and Bishwo. Of course, we also decorate any passers-by, so now there's a lot of people in the science building in Antarctica that are dressed for a luau!There's still a small chance we could get out to F6 later today. But the weather is not looking so good, which means we may be stuck in town until Monday. I'll keep you posted!

Nitrogen Fixation

Nitrogen is the most common element in the atmosphere and the air we breathe (78%), and it is a necessary element for life. It is an important element in proteins and enzymes, including the enzyme that allows plants to photosynthesize. However, the form of nitrogen that is in the air is not usable by plants. Plants use nitrogen compounds in the soil, not the nitrogen gas that is so abundant in the air. So how does all of that nitrogen from the air get into the soil for plants to use?

There are some organisms called cyanobacteria that can use the nitrogen gas in the air. They have special cells (called heterocysts) that can can grab nitrogen gas from the air and change it into a usable compound. This is a process called "nitrogen fixation". The cyanobacteria use that new form of nitrogen to grow. Then, when the cyanobacteria dies and decays, that usable form of nitrogen is released in the soil for plants and animals to use.

We see a lot of cyanobacteria near the streams and lakes in the dry valleys. It will grow in thick mats, like you see here. All of that black stuff is a bunch of cyanobacteria matted together, making a layer on top of the soil. One of the things we want to know is how much nitrogen the cyanobacteria are fixing. That way we can estimate how much nitrogen they are adding to the soil for the mosses and other soil animals to use. Unfortunately, it is not very easy to measure nitrogen fixation. But, we can measure a similar chemical process called "acetylene reduction" that helps us calculate how much nitrogen is being taken from the air. To measure acetylene reduction, we place small amounts of cyanobacteria (and mosses with cyanobacteria growing on it) into air-tight chambers, like the clear plastic ones in this picture. We then add a gas called acetylene to the chamber. The cyanobacteria will change the acetylene to ethylene in a process similar to how they change nitrogen gas. We leave the pieces of cyanobacteria and moss in the chambers for 1-5 hours. Then, we take a sample of the air inside the chamber and put it in a special vial. We then measure the amount of acetylene that has been changed to ethylene, and we can then calculate how much nitrogen would have been fixed from the air.

Here's Elizabeth and Ross measuring acetylene reduction at one of our field sites. Elizabeth is using a big syringe to pull new acetylene from a bag to inject into the plastic chambers. Ross is using another syringe to remove a gas sample from a chamber that contains some cyanobacteria.
We will take the vials of gas samples back to Dartmouth and measure the concentration of ethylene on one of the machines in our lab at home. So, we're doing the work now, but won't see the results for another month!

Dry Valley Wildlife

Most people think of Antarctica as being a big, cold, dead place. When you see photos of our research in the dry valleys, you don't see animals. But, there are animals there, in every single one of those pictures! You just can't see them, because they're microscopic. Here's some information about the main types of micro-organisms that live in Antarctic soils.

Nematodes
Also called roundworms, nematodes live EVERYWHERE in the world. You can find them in every biome in the world. They live in water, soil, ice, even in other animals! They are the most abundant animal in the world. In the dry valleys, we find more nematodes than any other animal. They are what our collaborators at Colorado State focus on studying. We have three main species that live here. The most numerous species, Scottnema lindsayae, is in the photo to the left.

Nematodes eat a lot of different things. Some, like the guy at the left, eat bacteria. Others eat fungi or algae, and some are even predators that eat other microscopic soil organisms. In the dry valleys, a predatory nematode is the top of the food chain! Though nematodes are small, they have a lot of very well-defined characteristics. Here is a very close-up picture of the mouth-parts of a bacterial-feeding nematode. They use those long branches on their mouths to scrape bacteria off of soil surfaces.

Rotifers
Rotifers also live in a lot of different environments, including fresh water, saltwater, soils, and other watery environments. A rotifer eats by waving the hairs around its mouth (at the top, in this picture) to catch things floating in the water. The moving action around their mouth looks kind of like a wheel, which is how they got the name ROTifer (like rotate). The "foot" (at the bottom of the picture) is to anchor the rotifer when it doesn't want to move.

Tardigrades
Tardigrades are also called water bears, and looking at the picture I think you can see why! They even have claws at the end of their feet, which you can see in this very close-up microscrope picture that Uffe took of a tardigrade foot. The claws let them hold on to something as they float through water or the water-filled spaces in the soil. Tardigrades eat with a stylet that they use to pierce animal and plant cell walls.

Tardigrades are able to live in a lot of extreme environments, and are found everwhere from the Himalayas to the ocean floor to Antarctica. They can withstand the pressure of a vaccuum, radiation, dehydration, and both incredibly high and low temperatures. There are even experiments that test tardigrades' ability to live in open space! In the dry valleys, they especially like to live in the moss and algae patches, where food and water are readily available.

The soil environment in the dry valleys is a very hard place to be an animal. There's not a lot of water, not a lot to eat, and it is very cold! Most of these micro-organisms have a special ability to help survive in such a harsh environment. They can go into anhydrobiosis, which essentially means they can freeze-dry themselves. They can push out all of their water and curl up, so that they don't freeze and die. Their metabolism drops to almost a stand-still! They can stay in anhydrobiosis for a very long time, and immediately wake up if water becomes available.

Part of our research looks at what animals are living in the soil. We want to know why they live in some places in the dry valleys, but not others. We want to know how nutrient cycles in the soil change when animals are there or not. So, we spend a lot of time on the microscopes looking at the animals we can extract from the soil. One of our team members, Byron, made this awesome video through the 'scope featuring three of our soil superstars: nematodes, rotifers, and tardigrades.



[Photos of each are from the linked websites: S. lindsayae, rotifer, tardigrade. Video by Byron Adams. Tardigrade foot photo taken by Uffe Nielsen.]

Tracer Experiment

Well, we finally made it out to the field. The weather at McMurdo has been very patchy. There's been snow and a lot of clouds. But, the weather out in the Dry Valleys has been very nice. So, once they finally had a short window of time to get us out, we left McMurdo and have been staying in the Valleys ever since in some beautiful, sunny weather. Katie and Elizabeth made it out to Lake Fryxell on Wednesday afternoon. Ross and I made it out to Lake Bonney on Thursday afternoon.

At Lake Bonney, Ross and I were working on an experiment that traced the movement of water from melting permafrost flowing over the soil. Permafrost is soil and rock in the ground that stays permanently frozen year-round. It usually is found below the soil surface in cold places like Antarctica and the Arctic. During the summer in the dry valleys, the permafrost will melt a little bit, causing wet patches to appear in the soil. You can see those darker, wet patches of soil in this picture at Bonney:Because it's been so warm this year, the permafrost below the soil has been melting a lot more than usual. At one of our research sites at Bonney, that meltwater has reach the surface (kind of like a spring you might find in the mountains in the US). There's so much meltwater that it ended up forming a stream that flows all the way down to the lake, straight through some long-term sampling plots we set up years ago! (Our team nickname in McMurdo is the Wormherders. Because the stream flows through one of our plots, it is called Wormherder Creek.) Instead of being upset that our plots are ruined, we're using the opportunity to learn about sources of water in the dry valleys and how they influence the soil.

On Thursday, we soil ecologists teamed up with a group of stream ecologists to study how the new Wormherder Creek interacts with the soil to influence soil chemistry and organisms. We added chemicals containing lithium and bromide high up on Wormherder Creek, and we followed how those chemicals moved down the stream and through the soil. There were two people at the top of the creek at the chemical injection site running the pump. Then, there were scientists stationed at regular intervals down the stream collecting samples every 5 or 10 minutes. I was stationed higher up on the creek with Uffe. In this video, you see Uffe labeling the bottle to take the next sample. That bottle gets dipped into the stream flow to be filled with water. You can see another scientist, Anna, further down the stream at the next station. You can also see how fast that meltwater is moving! It's a small creek, but there's a good bit of water in it! The tube next to the orange flag is called a piezometer, which is how we extract ground water from below the surface.

We collected a lot of surface water, ground water, and soil samples for several hours through the night. We will look for the presence and concentration of the chemicals we released high up in the stream so that we can follow how the water moved down to the lake. We will also measure the chemistry of the water and soil, and look at what kinds of organisms we find in various locations, to see how those important properties are influenced by the presence of Wormherder Creek. It was a loooong night, but we finished it!

After we left Lake Bonney late Thursday night, Ross headed to Fryxell to be with Katie and Elizabeth. I have been back at F6 (across the lake from the rest of the group) working on the stoichiometry plots that we treated last week. Hopefully I will be heading back to Lake Bonney tomorrow, but the weather does not look good in McMurdo, so the helicopters may not be able to come get me! Keep your fingers crossed, because I have samples to get back to the lab!

My bags are packed, I'm ready to go....

Because the Dry Valleys are so remote, we fly on helicopters to get there. Today we were supposed to fly to Lake Fryxell and Lake Bonney. But the weather is very bad for flying right now so we can't go anywhere. Helicopters only fly when the sky is clear and the wind is not blowing too hard. The helicopters here at McMurdo have lots of jobs to do, such as taking people to different places, delivering packages to field camps, and moving large pieces of equipment. Even though the helicopters have many jobs to do, safety is more important than everything else. So if the weather is bad enough, they all stay on the ground. Usually from the helicopter pad you can look out at the Ross Sea and also see the mainland on the other side. As this picture shows, you can't see much of anything this morning.

Last night we got a few inches of snow in McMurdo, even though it's the middle of summer!

We come down to Antarctic with plans to do lots and lots of scientific research, the weather is unpredictable. Sometimes we simply can't get out to our field locations and must wait for the weather to clear. So that's what we're doing today... waiting for better weather!

In and Back Out Again

Our final team member, Ross, arrived Saturday night. So, we are now a complete group of 4 from Dartmouth! Together, we've spent the past couple of days in McMurdo in our lab processing our samples.

Katie is working on a procedure called Chloroform Fumigation Extraction. For that, she is measuring the amount of carbon in the soil that is released after adding chloroform. Chloroform is a chemical that breaks open cells of living organisms (including people). So, if the carbon is released by the chloroform, it was released by the microbes living in the soil. Measuring the amount of carbon released by chloroform gives us an estimate of the microbial biomass living in the soil we are studying. Of course, Katie has to be careful not to breathe the chloroform herself. (Don't worry: when this picture was taken, she was already finished with the chloroform step!)
Elizabeth is busy picking all of the rocks out of her soil samples. The soils she is studying are very rocky, and the pebbles have to be removed before she can measure their chemistry. Doesn't that look like fun?!

We will be heading out to the field again tomorrow. We will be splitting up into two groups so that we can get more work done. Katie and Elizabeth will be going back to Lake Fryxell where we went the first week of our field work. Ross and I will be moving around all over the valley for the rest of the week. We'll be busy!

~~~~~~~~~~~~On another note~~~~~~~~~~~~~
Check out the awesome card I got in the mail yesterday, drawn by my friend Bob! Cedar waxwings like these live in the trees outside my office back at Dartmouth, so I put the card over my desk here in McMurdo to remind me of home.

Now where was that?

The plants and animals that live in the Dry Valleys are very small. Because they are so small, they are often difficult to locate, and even more difficult to find a second time.

This picture shows how closely you have to look to find the mosses that we study.

There are also no road signs pointing us in the direction of our research sites. We study certain spots in the field during one research season and try to get back to those same locations the next year. This is challenging because there are very few land features that we can use to remember a certain location and some things change from year to year. The streams are fed by melted snow, so they change depending upon how much snow falls during the winter and melts in the summer. This stream might have looked completely different last year, so finding a particular bend in the stream is nearly impossible.

Because of these navigational challenges, we use a GPS to help us find places in the Dry Valleys.
GPS stands for Global Positioning System. It uses satellites orbiting the earth that transmit signals, which enable GPS receivers to determine their current location. A GPS tells you your location in latitude and longitude coordinates. When we want to be able to find a certain spot again, we record the latitude and longitude coordinates so that we can later return to that exact location using the GPS.

Here's a picture of Elizabeth using a GPS to record the coordinates of a study site. If she wants to come back later, all she has to do is tell the GPS to find those coordinates and it will point an arrow in the direction we need to walk to get there. It also tells you distance.

A GPS isn't perfect. Sometimes we have a really hard time finding a spot using the coordinates a GPS gives us. It is often about 10 meters off, which is a lot when your study subject is only 1 centimeter tall. But the GPS is a very useful tool and we are very happy that it's in our scientific toolbox!